Our new Very clear+ tissue clearing strategy is the only real process that delipidates samples with no modify in morphology and with nominal effect on structural integrity.
Megatome is usually a vibrating microtome designed to segment a broad variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome allows superior-throughput tissue sectioning with uniform surface area profile, along with nominal tissue hurt and knowledge decline.
Antibodies may possibly just take months to diffuse by way of just a few millimeters of tissue, by using a steep labeling gradient from floor to Main.
Megatome is created for precision: the blade vibrates at a better frequency and larger amplitude assortment than other microtomes, and includes a special deflection Handle mechanism.
SE employs a rotational electrical area to disperse extremely electromobile molecules (such as antibodies or surfactant micelles) all over a porous sample without the need of detrimental electrically charged structures within the tissue. This allows two-four day clearing of intact organs,
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Megatome can be a novel microtome that allows for top-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with small tissue harm and knowledge reduction.
eFLASH is a swift tissue labeling approach which allows for uniform complete-organ staining in 20 rounds of labeling.
The SmartSPIM light sheet microscope megatomi.com offers unparalleled resolution and acquisition velocity during intact tissue volumes, while its modular layout allows you to up grade and customize particular person components.
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Protect avoids the variability of hydrogel embedding and the data loss from PFA preservation, safeguarding specimens for various rounds of processing.
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Our novel Defend tissue preservation procedure sorts intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.